Imiquimod Treatment Causes Systemic Disease in Mice Resembling Generalized Pustular Psoriasis in an IL-1 and IL-36 Dependent Manner

Figure 4

Systemic disease caused by imiquimod cream is IL-36α and IL-1R1 signaling dependent. Wild type (open bars, black circles, and lines), IL-36α^−/− (red bars, triangles, lines, and statistics asterisks), IL-1R1^-/- (blue bars, diamonds, lines, and statistics asterisks), and IL-36α^−/−/IL-1R1^−/− (purple bars, squares, lines, and statistics asterisks) female mice ( 3–5 per group) were treated daily with 5% imiquimod cream (Perrigo) for 4 days. Activity (a), nest building (b), posture (c), nose bulging (d), orbital tightening (e), weight (f), food intake (g), and survival (h) were evaluated and scored as described in Table 1 and illustrated in Figure 2. ND: no disease detected, score = 0. Total disease scores (i) were calculated as the sum of individual scores for each mouse and group means (SD) shown. ; ; (compared to control at individual timepoints unless indicated otherwise with black bars). Data is shown from one representative experiment of 3 independent experiments.

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