Research Article
Imiquimod Treatment Causes Systemic Disease in Mice Resembling Generalized Pustular Psoriasis in an IL-1 and IL-36 Dependent Manner
| Phenotype | Readout | Outcome | Score |
| Anorexia | Weight | No change or increase in weight | 0 | 1–5% decrease in weight | 1 | 6–10% decrease in weight | 2 | 11–15% decrease in weight | 3 | >15% decrease in weight | 4 | Food intake | 10–15 g of diet eaten | 0 | 3–9 g of diet eaten | 1 | 0–2 g of diet eaten | 2 |
| Malaise | Nest building | Nestlet shredded 80–100% | 0 | Nestlet shredded 20–79% | 1 | Nestlet shredded 0–19% | 2 | Activity | Mouse runs away when hand is placed behind it to pick it up by the tail | 0 | Mouse moves away when hand is placed behind it to pick it up by the tail | 1 | Mouse is reluctant to move even when touched | 2 | Mouse is found dead | 3 |
| Pain | Posture | Normal gait | 0 | Modestly curved back when walking | 1 | Back curved and moves with front and hind legs closely together | 2 | Nose bulge | Head is pointy and hair from the nose to the ears is lying flat | 0 | Hair from the nose to the ears is slightly raised | 1 | Hair from the nose to the ears is standing up giving the appearance of a different head shape | 2 | Orbital tightening | Eyes are open and nearly circular | 0 | Slight squint | 1 | Eyes nearly closed | 2 |
| Skin inflammation | Appearance | Skin looks normal | 0 | Skin appears dry | 1 | Skin is red and flaky | 2 |
|
|