Imiquimod Treatment Causes Systemic Disease in Mice Resembling Generalized Pustular Psoriasis in an IL-1 and IL-36 Dependent Manner

Table 1

Disease scoring.

Phenotype	Readout	Outcome	Score
Anorexia	Weight	No change or increase in weight	0
		1–5% decrease in weight	1
		6–10% decrease in weight	2
		11–15% decrease in weight	3
		>15% decrease in weight	4
	Food intake	10–15 g of diet eaten	0
		3–9 g of diet eaten	1
		0–2 g of diet eaten	2
Malaise	Nest building	Nestlet shredded 80–100%	0
		Nestlet shredded 20–79%	1
		Nestlet shredded 0–19%	2
	Activity	Mouse runs away when hand is placed behind it to pick it up by the tail	0
		Mouse moves away when hand is placed behind it to pick it up by the tail	1
		Mouse is reluctant to move even when touched	2
		Mouse is found dead	3
Pain	Posture	Normal gait	0
		Modestly curved back when walking	1
		Back curved and moves with front and hind legs closely together	2
	Nose bulge	Head is pointy and hair from the nose to the ears is lying flat	0
		Hair from the nose to the ears is slightly raised	1
		Hair from the nose to the ears is standing up giving the appearance of a different head shape	2
	Orbital tightening	Eyes are open and nearly circular	0
		Slight squint	1
		Eyes nearly closed	2
Skin inflammation	Appearance	Skin looks normal	0
		Skin appears dry	1
		Skin is red and flaky	2