Exploring New Inflammatory Biomarkers and Pathways during LPS-Induced M1 Polarization

Figure 3

M1 polarized N9 microglia have reduced ability to migrate towards ATP and lipopolysaccharide (LPS). Migration was assessed using a Boyden Chamber. Nontreated (control) and LPS-stimulated microglia were allowed to migrate for 6 h to basal medium (basal migration), ATP at 10 μM and LPS at 300 ng/mL. Representative results of one experiment are shown (a). The total number of cells per well was counted and the results were expressed as fold versus control (basal migration) (b). Results are mean ± s.e.m. from four independent experiments. Comparison was done by one-way ANOVA followed by multiple comparisons Bonferroni post hoc correction. and versus respective control; versus basal migration in controls; and versus migration of control cells to ATP. Scale bar represents 100 μm.

(a)

(b)