Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells

Figure 3

Expression pattern of TLR and selectin cascade genes in HAECs stimulated by SAA. Expression levels of TLR2, MYD88, NFKB1, TNF-α, and SELE mRNAs were examined at 0, 1, 3, and 6 h in HAECs stimulated by SAA. The mRNA expression of TLR2 was induced by SAA (closed column) in a time-dependent manner. Furthermore, the mRNA expression of NFKB1, TNF-α, and SELE was significantly upregulated by SAA (closed column) compared with the control (open column). Data represent the mean ± SD of four independent experiments. Each experiment was performed in triplicate. , , and . rhSAA: recombinant human SAA.