Metallothioneins 1 and 2 Modulate Inflammation and Support Remodeling in Ischemic Cardiomyopathy in Mice

Figure 2

Increased cardiomyocyte apoptosis in M-hearts. Representative slides of TUNEL stained sections present (a) only few apoptotic nuclei in cardiomyocytes of WT-hearts, when compared to (b) numerous nuclei with DNA-fragmentation in M-hearts after 3 days of (d) . (c) TUNEL-positive cells after 3 days of . RT-qPCR showing mRNA expression of (d) caspase- (Casp-) 8, (e) caspase- (Casp-) 3, and (f) B-cell lymphoma (Bcl)-2 during repetitive . = 8–10/group; scale bar in (a) and (b): 75 μm; RT-qPCR using Taqman, mRNA expression is related to shams and GAPDH using comparative ΔΔCt-method; indicates P ≤ 0.05 between the genotypes; # indicates P ≤ 0.05 versus respective sham.

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