Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy

Figure 5

Effects of ASPP2 on the protein expression of EMT-related cytokines of ARPE-19 cells. After transfection with either scrambled control-siRNA or ASPP2-siRNA for 48 hours, protein levels of ARPE-19 cells were analyzed by western blot using antibodies against TGF- and CTGF (a). -actin was used as a loading control. Representative results obtained in three independent experiments are shown. Column diagrams and bars represent the ratio of the scanned immunoblots of TGF- and CTGF to -actin (b). SC was set to 100%. Meanwhile, concentrations of IL-8, IL-6, and VEGF in the culture supernatants of ARPE-19 cells were quantified with the CBA assay (c). Data are shown as the mean ± SD, experiments. versus SC group. SC, scrambled control-siRNA-transfected cells; ASPP2-si, ASPP2-siRNA-transfected cells.

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