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Mediators of Inflammation
Volume 2016, Article ID 7937814, 8 pages
Research Article

DNA Damage in CD133-Positive Cells in Barrett’s Esophagus and Esophageal Adenocarcinoma

1Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
2Faculty of Nursing Science, Suzuka University of Medical Science, Suzuka, Mie 513-8670, Japan
3Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan
4Division of Gastroenterology, Tohoku University Hospital, Sendai 980-8574, Japan
5Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka, Mie 513-8670, Japan

Received 18 November 2015; Revised 21 January 2016; Accepted 17 February 2016

Academic Editor: Hamid Boulares

Copyright © 2016 Raynoo Thanan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Immunohistochemical study of phosphorylated H2AX: Phosphorylated H2AX (γ-H2AX) was stained by using mouse monoclonal anti-phospho-H2AX antibody (clone JBW301, 4 μg/mL, Merck Milipore, Darmstadt, Germany) as a primary antibody. Paraffin sections were incubated with the primary antibodies overnight at room temperature. The sections were next incubated with fluorescent secondary antibody (1:400 Alexa 488-labeled goat anti-mouse IgG (Molecular Probes Inc., Eugene, Oregon, USA) for 3 h at room temperature. Finally, the nuclei were stained by 4'-6-diamidino-2-phenylindole (DAPI) and the sections were examined with a fluorescence microscope (LX70, Olympus, Tokyo, Japan) ora laser scanning confocal microscope (Fluoview FV1000-D, Olympus). For the negative control, we omitted the primary antibody and treated with the secondary antibody according to the procedure.

  1. Supplementary Material