Effects of Chinese Propolis in Protecting Bovine Mammary Epithelial Cells against Mastitis Pathogens-Induced Cell Damage

Table 1

Sequences of primers used for quantitative real-time RT-PCR.

Gene	Primer sequence		Product size (bp)	GenBank accession number
IL-6	F	5′-AAACGAGTGGGTAAAGAACGC-3′	143	NM_173923.2
	R	5′-GACCAGAGGAGGGAATGCC-3′
IL-8	F	5′-TCTCAGCCATCTTTTTACCTCAC-3′	176	NM_173925.2
	R	5′-ATCAACATTCTTTCCCATTTCTC-3′
TNF-α	F	5′-CGGTGGTGGGACTCGTA-3′	185	NM_173966.3
	R	5′-AATGCGGCTGATGGTGT-3′
HO-1	F	5′-GGCAGCAAGGTGCAAGA-3′	221	NM_001014912.1
	R	5′-GAAGGAAGCCAGCCAAGAG-3′
Txnrd1	F	5′-GTGTTCACGACTCTGTCGGT-3′	240	NM_174625.3
	R	5′-CTGCCTTCCACGAATCACCT-3′
GCLM	F	5′-GACAAAACCCAGTTGGAGCA-3′	235	NM_001038143.1
	R	5′-AGTACCGCAGTAGCCACAGAG-3′
β-actin	F	5′-CAAGGACCTCTACGCCAAC-3′	257	NM_173979.3
	R	5′-AGAAGCATTTGCGGTGGAC-3′

F = forward; R = reverse.