Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages

Figure 4

Comparative effects of LDLs on (un)polarized RAW 264.7 macrophages. M0, M1, and M2 macrophages were stimulated in the presence or absence (RPMI control) of Nat-LDLs, Ox-LDLs, and MpOx-LDLs for 24 hours (100 μg/mL). (a) Expression of polarization marker genes at the mRNA level (RT-qPCR). The expression of marker genes was analyzed by RT-qPCR as described in Figure 1. Data are normalized with TBP used as housekeeping gene and expressed as mean fold induction relatively to M0 cells in RPMI ± SD (). Data for MRC1 were analyzed by a Kruskal-Wallis ANOVA on ranks. Zoomed data for Arg1 and MRC1 are presented in Figure S5. (b). Expression of polarization marker genes at the protein level (ELISA; WB). (b1) Production of secreted IL-6 (M1 marker) in cell culture supernatants assessed by ELISA. Data are expressed relatively per μg of protein per well as mean ± SD (). (b2) Expression of HO-1 and Srxn1 assessed by Western blotting. Data are normalized with TBP used as loading control and expressed as mean ± SD (). Results are representative of 3 independent experiments. ANOVA 2: ;   ; and .