Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages
Comparative effects of LDLs on (un)polarized BMDMs. M0, M1, and M2 macrophages were stimulated in the presence or absence (RPMI control) of Nat-LDLs, Ox-LDLs, and MpOx-LDLs for 24 hours (100 μg/mL). (a) Expression of polarization marker genes at the mRNA level (RT-qPCR). The expression of marker genes was analyzed by RT-qPCR as described in the preceding figures. Data are normalized with TBP used as housekeeping gene and expressed as mean fold induction relatively to M0 cells in RPMI ± SD (). Data were analyzed by a Kruskal-Wallis ANOVA on ranks (except for Arg1 and IL-6: two-way ANOVA test). (b) Expression of polarization markers at the protein level (ELISA; WB). (b1) Production of secreted IL-6 (M1 marker) in cell culture supernatants assessed by ELISA. Data are expressed relatively per μg of protein per well as mean ± SD (). (b2) Expression of HO-1 and Srxn1 assessed by Western blotting. Data are normalized with TBP used as loading control and expressed as mean ± SD (). Results are representative of 4 independent experiments. ANOVA 2: ; ; and ."
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