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Mediators of Inflammation
Volume 2016, Article ID 8249476, 20 pages
Research Article

Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages

1URBC-Narilis, University of Namur, 61 rue de Bruxelles, 5000 Namur, Belgium
2UMBD, University of Namur, 61 rue de Bruxelles, 5000 Namur, Belgium
3Laboratory of Experimental Medicine (ULB 222 Unit), Université Libre de Bruxelles, CHU-Charleroi, ISPPC Hôpital Vésale, Montigny-Le-Tilleul, Belgium
4Therapeutic Chemistry, ULB (Campus de la Plaine) CP205/05, boulevard du Triomphe, Brussels, Belgium

Received 25 April 2016; Revised 28 July 2016; Accepted 2 August 2016

Academic Editor: Michal A. Rahat

Copyright © 2016 Valérie Pireaux et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figures support our data showing an overexpression of M2 markers at the mRNA level (Mgl2, YM1, Arg1 and MRC1) (Figures S1 and S5) and at the protein level (IL-10) (Figure S3), when M0, M1 and M2 macrophages are stimulated with MpOx-LDLs. There is no marked effect of MpOx-LDLs on M1 markers at the mRNA level (Arg2 and TNF-α) (Figure S2) and at the protein level (IL-12 and TNF-α) (Figure S3). Figure S4 shows the effects of LDLs on the secretion of cytokines (IL-10, IL-12 and TNF-α) in RAW 264.7 M0 macrophages stimulated with Nat-LDLs, Ox-LDLs or MpOx-LDLs for 24 hours. Figure S6 shows the impact of native or oxidized LDLs on the phagocytosis of fluorescent beads by (un)polarized RAW 264.7 macrophages and BMDMs. Materials and methods are described in the Materials and Methods section of the manuscript.

  1. Supplementary Material