Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells

Figure 4

Effect of HMW-HA, MMW-HA, and LMW-HA in RPMI 2650 cells stimulated with rhIL-17A. (a) The cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 6 hrs. ROS production was evaluated in the cells by flow cytometry. The bars expressed mean ± SD of 3 separate experiments. Representative flow cytometry is shown; (b) the cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 18 hrs. NOX-4 and IL-8 protein synthesis were evaluated in the cell lysates by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative western blot is shown. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

(a)

(b)