Disruption of Tumor Necrosis Factor Receptor-Associated Factor 5 Exacerbates Murine Experimental Colitis via Regulating T Helper Cell-Mediated Inflammation

Figure 5

Increased proportions of Th2 and IFN-γ/IL-17a-coproducing CD4⁺ T cells in the colons of TRAF5-deficient animals induced by DSS. LPMCs obtained from the colons of DSS-fed KO and WT mice were stimulated with PMA/ionomycin and subjected to surface staining for CD4. After fixation and permeabilization, intracellular staining for IL-4, IFN-γ, and IL-17a was performed as described in Section 2. CD4⁺ T cells were then gated and analyzed. (a) Representative flow plots. The percentages of (b) Th2 cells (CD4⁺IL-4⁺), (c) IFN-γ/IL-17a-coproducing CD4⁺ T cells (CD4⁺IFN-γ⁺IL-17a⁺), (d) classical Th1 cells (CD4⁺IFN-γ⁺IL-17a⁻), and (e) classical Th17 cells (CD4⁺IL-17a⁺IFN-γ⁻) are presented as the mean ± SD. The data are representative of 3 independent experiments. per group. versus the DSS-treated WT group. NS: not significant versus the DSS-treated WT group.

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