Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

Figure 7

Effect of ER stress-induced tunicamycin on the antioxidant expression profile in mouse precision-cut lung slices (PCLS). PCLS were obtained from wild type mice and were exposed to tunicamycin (1 μM) for 24 hours. (a) LDH release into media and (b) Chop, Atf4, Atf6, and Xbp1 gene expression were examined. (c) Immunoblots were conducted for Chop, Atf4, Atf6, Xbp1, andβ-actin. (d) GPx-1, GPx-2, GPx-3, GPx-4, and Sod1 were quantified by qPCR and (e) immunoblots analysis. Every lane represents an individual mouse. Dot plots are represented as relative quantification (RQ) compared to ACTB expression or densitometry units (DU) of pixel intensity expressed as a ratio to β-actin. Data are shown as the mean ± SEM, where each measurement was performed on 3 independent days on 6 donors/group. denotes a value < 0.05, when comparing both treatments connected by a line, determined by Student’s -test (2 groups).

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