Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis

Figure 5

Effects of IL-29 on cartilage degradation by OA FLS ex vivo. (a) The OA cartilage depletion after being cocultured with OA FLS for 72 h was visualized in Safranin O/Fast Green-stained sections. Representative images from one experiment are reported (a), and the reduced intensity of red stain denotes proteoglycan loss (original magnification ×100). (b) The depth of GAG depletion (μm) in cartilage was measured from the articular surface to the red/orange tidemark (marked by arrow). (c) The ratio of MM-1, MM-2, MM-3, and MM-13 to TIMP-1 was determined by real-time PCR in OA FLS. (d) MMP-3 in culture supernatants harvested from each well after 72 h was measured by ELISA. The error bars represent mean ± SEM for triplicate experiments. versus medium control. versus medium control.

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