Identification of a Novel lincRNA-p21-miR-181b-PTEN Signaling Cascade in Liver Fibrosis
miR-181b is involved in the effects of lincRNA-p21 on PTEN expression and HSC activation. Cells were transduced with Ad-lincRNA-p21 for 48 h and treated with miR-181b mimics for additional 48 h. (a) Expressions of miR-21, miR-24, miR-32, miR-93, miR-153, miR-181b, miR-205, and miR-214 were detected in cells transduced with lincRNA-p21. (b) Expression of miR-181b in liver tissues of healthy controls () and cirrhotic patients (). (c) Expression of miR-181b in miR-181b mimics group. (d) lincRNA-p21-induced PTEN was inhibited by miR-181b. (e) The effect of Ad-lincRNA-p21 on cell proliferation was suppressed by miR-181b. (f) The reduced mRNA expressions of α-SMA and Col1A1 by Ad-lincRNA-p21 were inhibited by miR-181b. (g) The reduced protein expressions of α-SMA and type I collagen by Ad-lincRNA-p21 were inhibited by miR-181b. GAPDH was used as internal control. Each value is the mean ± SD of three experiments. compared with the control and compared with Ad-lincRNA-p21 group.
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