Research Article

Apoptosis of Endothelial Cells by 13-HPODE Contributes to Impairment of Endothelial Barrier Integrity

Figure 3

Mean changes in mRNA expression of bovine monocyte oxylipid biosynthetic enzymes (a, b), a marker of monocyte activation (c), and inflammatory cytokines (d, e). Media controls are displayed as open bars. Positive control is 4 hr LPS and displayed in closed bars. Light grey bars represent S. uberis supernatant exposure for 4 hr and 36 hr. Dark grey bars represent heat-killed S. uberis exposure for 4 hr and 36 hr. The mRNA expression is expressed as    SE. Asterisks () denote differences between media control and positive control (LPS) as tested by Student’s t-tests. Letters that differ between control time points, 4 hr and 36 hr separately, denote significant differences between control and among treatments as measured by an ordinary one-way ANOVA with Tukey’s post hoc correction. For example, 4 hr exposure to heat-killed S. uberis upregulated COX-2 mRNA expression compared to control but 4 hr exposure to S. uberis supernatant did not (a). Additionally, COX-2 mRNA expression after heat-killed S. uberis exposure is not different from COX-2 mRNA expression after S. uberis supernatant exposure (a). A similar relationship can be described for 36 hr exposure of bovine monocytes to heat-killed and S. uberis supernatant. Significance declared for all tests using ΔCt at P ≤ 0.05 ( for 15-LOX-1, IL-6, and IL-10; for COX-2 and iNOS).