Apoptosis of Endothelial Cells by 13-HPODE Contributes to Impairment of Endothelial Barrier Integrity
Monocyte oxylipid biosynthesis following 4 hr LPS (black bars) or S. uberis exposure. Media controls are displayed as open bars. Light grey bars represent S. uberis supernatant exposure for 4 hr and 36 hr. Dark grey bars represent heat-killed S. uberis exposure for 4 hr and 36 hr. Mean oxylipid biosynthesis is expressed as ng of oxylipid/mL of media. Letters that differ between control time points, 4 hr and 36 hr separately, denote significant differences between control and among treatments as measured by an ordinary one-way ANOVA with Tukey’s post hoc correction. For example, 4 hr exposure to S. uberis supernatant or heat-killed S. uberis did not modify 13-HODE biosynthesis, whereas 36 hr exposure to heat-killed S. uberis did increase 13-HODE biosynthesis. No letters are displayed in (b) as no differences were detected in any time points. Significance for differences declared at ().