ZNF395 Is an Activator of a Subset of IFN-Stimulated Genes

Figure 4

Activation of CXCL10 and CXCL11 expression by ZNF395 requires active IKK. RTS3b-TR-FLAG-ZNF395-2 cells were treated with Dox to induce the expression of ZNF395, with IFNα and the IKKβ-specific inhibitor BMS-345541 in various combinations as indicated in the figure. QRT-PCR was performed to determine the level of transcripts for CXCL10, CXCL11, and ZNF395. After normalization with the house keeping gene HPRT, the values obtained with untreated cells were set as 1 and fold changes upon the different treatments were calculated by Δ-threshold method. The diagrams on the right are provided for a better resolution. The graphs represent the results of three qRT-PCRs with RNA obtained from one experiment. The results have been confirmed with RNA from an independent second experiment (data not shown).