Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis

Figure 3

Comparison of L2-MHV3 viral nucleocapsid, mRNA expression of viral recognition receptors, and IFN-β expression in WT and IL-33 KO mice. Total liver RNA was extracted from WT and IL-33 KO mice control (PBS) or L2-MHV3-infected mice (48 h and 72 h PI) and tested by RT-qPCR for (a) L2-MHV3 nucleocapsid viral RNA expression, (b) CEACAM1 mRNA, (c) TLR2 mRNA, (d) TLR3 mRNA, and (e) IFN-β mRNA. The mean expression of the WT PBS mice was used as a control and arbitrarily considered as 1 AU (arbitrary unit) which served as a reference for fold change in other conditions. Statistical analyses were done according to the Mann–Whitney U test. # represents significant difference with WT PBS mice, represents significant difference between WT and IL-33 KO conditions, and $ represents significant difference between two conditions in the same background mice. ^#; ^##; and ^###.

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