Research Article
Deregulation of Regulatory T Cells in Acute-on-Chronic Liver Failure: A Rat Model
Figure 1
The process of making models of ACLF and ALF. All rats were classified into two groups randomly: ALF group and ACLF group. (1) ACLF group: the rats were treated with CCl4 dissolved in peanut oil (volume, 1 : 1) 1.5 mL/Kg weight in the 1st month and 2.0 mL/Kg weight in the 2nd month once every three days; six rats were selected as the baseline control and named “0 h”; the rest rats were treated with 500 mg/Kg weight D-GalN and 80 μg/Kg weight LPS, and five time points were selected. (2) ALF group: no treatment was received for two months; subsequent treatments of ALF group were the same as ACLF group (ALF: acute hepatic failure; ACLF: acute-on-chronic liver failure; CCl4: carbon tetrachloride; D-GalN: D-galactosamine; LPS: lipopolysaccharide).