Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages

Figure 1

Piceatannol impacts IRAK1 activation during LPS treatment in RAW264.7 cells. (a, b, and c) Protein levels of IRAK1, IRAK4, MyD88, and β-actin as determined by lysate immunoblotting prepared from LPS- (1 μg/ml) treated RAW264.7 cells or MyD88-overexpressed HEK293 cells. (d and e) HEK293 cells cotransfected with NF-κB-Luc (1 μg/ml) and β-gal (as a transfection control) plasmid constructs were treated with piceatannol (40 μM) in the presence or absence of an adaptor molecule (MyD88) or IRAK1 for 12 h. Luciferase activity was determined via luminometry. All data are expressed as the mean ± SD of three independent experimental replicates. compared to the control group.

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