Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion

Figure 1

Regulation of constitutive CX3CL1 expression by shear stress. (a–c) HUVECs were cultured for 24 h under different conditions of flow resulting in the indicated laminar shear stress. Cells were then analyzed for their morphology by microscopy (a), for CX3CL1 mRNA expression (b), and their release of soluble CX3CL1 into the supernatant (c). (e, g) HUVECs were cultured under different flow conditions as described for (a–c) and analyzed for CXCL16 mRNA expression (e) and their release of soluble CXCL16 into the supernatant (g). (d, f) Endothelial cells from different vascular beds were cultured for 24 h with and without flow and analyzed for their CX3CL1 (d) or CXCL16 (f) mRNA expression. Data in (b–g) are shown as mean + SD for at least five different experiments. Statistical differences in comparison to the static control are indicated by asterisks (, ).

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