Involvement of Toll Like Receptor 2 Signaling in Secondary Injury during Experimental Diffuse Axonal Injury in Rats

Figure 9

Inhibition or activation of TLR2 was associated with glial reaction. (a) Marker of astrocyte vimentin, GFAP, and marker of microglial cells Iba-1 were assessed by immunohistochemistry in rat cortex (scale bar = 100 μm). (b) The bar graphs showed the statistical results for vimentin, GFAP, and Iba-1 assessed by immunohistochemistry. The relative increases in vimentin, GFAP, and Iba-1 levels were assessed by densitometry (IOD) and positive cell levels were calculated in 10 random fields (40x magnification). Data were presented as mean ± SD in three separate experiments ( = 6; < 0.05, compared with control group; < 0.05, compared with DAI 3 d group).

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