Research Article
The Astrocytic S100B Protein with Its Receptor RAGE Is Aberrantly Expressed in SOD1G93A Models, and Its Inhibition Decreases the Expression of Proinflammatory Genes
Figure 7
Reduction of proinflammatory genes expression by S100B silencing in SOD1G93A primary astrocytes. Primary astrocytes were transfected with scramble (si-scr) or anti-S100B siRNAs (si-S100B) at 24 and 48 h after plating. 72 hours after plating, cells were lysed, and RNA (a, c) or protein was extracted (b). (a, c) cDNA from si-scr and si-S100B-treated cells was analyzed by real-time qPCR for S100B, GFAP, TNFα, CXCL10, and CCL6 expression. The panels show the quantification of each mRNA expressed in arbitrary units (AU) and reported as mean ± s.d., relative to corresponding si-scr ( independent experiments). . (b) Western blot analysis with anti-S100B, anti-GFAP, and anti-GAPDH. In the right panels, the quantification of S100B (blue) and GFAP (red) bands normalized to GAPDH and relative to si-scr. Data are expressed as mean ± s.d. ( independent experiments). .
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