Evidence for the Involvement of Lipid Rafts and Plasma Membrane Sphingolipid Hydrolases in Pseudomonas aeruginosa Infection of Cystic Fibrosis Bronchial Epithelial Cells

Figure 12

Sphingolipids composition of DRM and HD fractions obtained from CuFi-1 cells, infected or not with P. aeruginosa. (a) HPTLC of radioactive lipids contained in the total lipid extract, and aqueous phase obtained from detergent-resistant membrane (DRM) and high-density (HD) fraction of CuFi-1 cells, infected or not with PAO-1 (left). Semiquantitative graph of ganglioside composition of DRM from both infected and noninfected CuFi-1 cells. Data are expressed as radioactivity associated with the aqueous phase (right). (b) HPTLC of radioactive lipids contained in the alkali-treated organic phase obtained from the detergent-resistant membrane (DRM) and high-density (HD) fraction of CuFi-1 cells, infected or not with PAO-1 (left). Semiquantitative graph of sphingolipid species contained in the organic phase of DRM and HD fraction obtained from both infected and noninfected CuFi-1 cells. Data are expressed as radioactivity associated with the organic phase. The same amount of fraction were loaded on HPTLC. Cer: ceramide; GlcCer: glucosylceramide; PE: phosphatidylethanolamine; Gb3: globotriaosylceramide; SM: sphingomyelin. versus CTRL.

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