The Combination of MBP and BCG-Induced Dendritic Cell Maturation through TLR2/TLR4 Promotes Th1 Activation In Vitro and Vivo

Figure 1

The activation of CD4⁺ T cells induced by the combination of MBP and BCG. (a) Isolation of CD4⁺ T cells from spleen samples from normal mice. The CD4⁺ T cell purity was analyzed by flow cytometry. (b) Dendritic cells were isolated from the spleen, and the percentage of dendritic cells purified was measured by FACS analysis. (c) MBP and BCG synergistically increased the proliferation of CD4⁺ T cells cocultured with DCs. All experiments were repeated three times, and all the data are expressed as the mean ± SD (). was considered significant, compared with Blank group or with MBP group. (d and e) The production of IFN-γ and IL-4 in CD4⁺ T cells cocultured with DCs, as detected by ELISPOT. CD4⁺ T cells were cocultured with DCs at a ratio of 100 : 1. (f and g) Spots were counted by an ELISPOT reader.

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