Research Article
The Combination of MBP and BCG-Induced Dendritic Cell Maturation through TLR2/TLR4 Promotes Th1 Activation In Vitro and Vivo
Figure 5
Effect of the combination of MBP and BCG on the phenotypic maturation of DCs from TLR2−/− mice. Splenic DCs from TLR2−/− mice (1 × 106 cells/mL) were cultured with MBP, BCG, or the combination of MBP and BCG for 48 h and were analyzed using flow cytometry. (a–c) DCs collected from different groups were stained with the following antibodies: FITC-conjugated anti-CD80, PE-conjugated anti-CD86, and APC-conjugated anti-MHC class II. The percentage of positive cells is shown in a flow cytometry histogram. (e–g) The percentage of CD11C+ cells expressing each surface molecule is expressed as the mean ± standard deviation and is shown in a bar graph. Data are derived from three independent experiments. is considered to indicate statistically significant differences, compared with control group derived from WT mice. (d) The mRNA level of TLR2 in the spleens of WT mice or TLR2−/− mice was detected with RT-PCR.
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