The Combination of MBP and BCG-Induced Dendritic Cell Maturation through TLR2/TLR4 Promotes Th1 Activation In Vitro and Vivo

Figure 7

The effect of the combination of MBP and BCG on the expression of the surface molecules TLR2 and TLR4 on DCs in vivo. DCs isolated from the spleens of normal mice immunized with MBP, BCG, or the combination of MBP and BCG were incubated with FITC-conjugated anti-TLR4, PE-conjugated anti-TLR2, and APC-conjugated anti-CD11c at 4°C for 30 min. Then, cells were analyzed for the expression of TLR2/TLR4 on DCs with flow cytometry. (a) TLR4 expression on DCs from mice immunized with MBP, BCG, or the combination of MBP and BCG. (b) The expression of TLR2 on DCs isolated from the spleens of mice immunized with MBP, BCG, or the combination of MBP and BCG. (c-d) The percentage of CD11c⁺ cells expressing the TLR2/TLR4 molecules is expressed as the mean ± standard deviation and is shown in a bar graph. Data are derived from three independent experiments. is considered to indicate statistically significant differences compared with the NS group.

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