Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands

Figure 4

Estrogen but not BPA upregulates the expression of costimulatory molecules upon stimulation with CpG and LPS. (a–i) We grew cDCs in the absence or presence of low (0.05 nM) and high doses (50 nM) of E2 or BPA for 6-7 days. We then stimulated cDCs with either 100 ng/mL of LPS (c; f; i) or 10 μg/mL of CpG (b; e; h) as described in Figure 2 and analyzed the percentages of cDCs (gated for CD11c+ cells) positive for the indicated costimulatory and MHC molecules, 24 hours post stimulation with TLR ligands. Results are averages and SE of four BMDC cultures. Dotted lines indicate baseline expression in unstimulated cDCs grown in the absence of E2 or BPA; pos indicates positive. For each costimulatory or MHC molecule, we calculated statistical significance by one-way ANOVA and post hoc multiple comparison test against 0 condition (no E2 nor BPA). represents , for , and for .

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