Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells

Figure 1

Nicotine stimulated the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun in RAW264.7 cells. (a) RAW264.7 cells were treated with 10 mg/ml nicotine for 0, 10, 20, 30, 60, and 120 min. (e) RAW264.7 cells were exposed to nicotine for 30 min at the concentrations of 0, 1, 10, and 100 mg/ml. Cell lysates were collected and protein levels of p-ERK1/2, ERK1/2, p-c-Jun, c-Jun, p-p65, and p65 were measured by Western blot. The band optical density values (means ± SD) of p-ERK1/2 (b, f), p-c-Jun (c, g), and p-p65 (d, h) were evaluated using ImageJ software, with all experiments being analyzed as three different independent experiments and GAPDH used as an internal control. and versus controls.

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