Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells

Figure 4

PNU-282987 suppressed nicotine-stimulated activation of extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun in RAW264.7 cells. (a) RAW264.7 cells were treated with 10 μm PNU-282987 for 0, 15, 30, 60, 90, and 120 min. (e) RAW264.7 cells were pretreated with 10 μm PNU-282987 for 60 min prior to 10 ng/ml nicotine exposure for 30 min. Protein levels of p-ERK1/2, ERK1/2, p-c-Jun, c-Jun, p-p65, and p65 were measured by Western blot, and the intensity of protein bands (means ± SD) (b–d, f, g) were assessed using ImageJ software, with all experiments being analyzed as three different independent experiments and GAPDH used as an internal control. and versus controls; ^# and ^## versus the group treated with nicotine.

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