Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells

Figure 6

PNU-282987 inhibited nicotine-stimulated activation of c-Jun in MOVAS cells. (a) MOVAS cells were exposed to 10 μm PNU-282987 for 0, 15, 30, 60, 90, and 120 min. (e) MOVAS cells were pretreated with 10 μm PNU-282987 for 60 min prior to 10 ng/ml nicotine exposure for 30 min. Cell lysates were collected, and protein levels of phosphorylated extracellular signal-regulated kinase1/2 (p-ERK1/2), ERK1/2, p-c-Jun, c-Jun, p-p65, and p65 were measured by Western blot. (b–d, f, g) Densitometric analysis of protein bands was performed via ImageJ software, with all experiments being analyzed as three different independent experiments and GAPDH used as an internal control. and versus controls; ^# and ^## versus the group treated with nicotine.

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