Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis

Figure 4

Buprenorphine administration altered serum cytokine production in the CIA mice. Serum was collected from the CIA mice with and without buprenorphine administration on day 29 after the first CII challenge (day of sacrifice). Circulating levels of a panel of cytokines (IFN-γ, IL-2, IL-6, TNF-α, IL-1β, and IL-10) were analyzed using the V-PLEX proinflammatory panel 1 (mouse) by multiplex Meso Scale Discovery (MSD) platform (Meso Scale Discovery, Rockville, MD, USA) employing the manufacturer’s protocol. The data was analyzed using Discovery Workbench 4.0 software (Meso Scale Discovery). Kruskal-Wallis one-way analysis of variance (ANOVA) followed by Dunn’s post hoc test was used to determine the significance, and Mann–Whitney U test was used to determine the values between the groups; was considered to be statistically significant; ns: not significant.