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Mediators of Inflammation
Volume 2017, Article ID 2810295, 7 pages
https://doi.org/10.1155/2017/2810295
Research Article

Effects of Src Kinase Inhibition on Expression of Protein Tyrosine Phosphatase 1B after Brain Hypoxia in a Piglet Animal Model

1Department of Pediatrics, Texas Tech University-HSC at the Permian Basin, Odessa, TX, USA
2Department of Pediatrics, Drexel University and St. Christopher’s Hospital for Children, Philadelphia, PA, USA

Correspondence should be addressed to Dimitrios Angelis; ude.cshutt@silegna.soirtimid

Received 26 February 2017; Accepted 13 April 2017; Published 23 May 2017

Academic Editor: Cristian Palmiere

Copyright © 2017 Dimitrios Angelis and Maria Delivoria-Papadopoulos This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Protein tyrosine phosphatases (PTPs) in conjunction with protein tyrosine kinases (PTKs) regulate cellular processes by posttranslational modifications of signal transduction proteins. PTP nonreceptor type 1B (PTP-1B) is an enzyme of the PTP family. We have previously shown that hypoxia induces an increase in activation of a class of nonreceptor PTK, the Src kinases. In the present study, we investigated the changes that occur in the expression of PTP-1B in the cytosolic component of the brain of newborn piglets acutely after hypoxia as well as long term for up to 2 weeks. Methods. Newborn piglets were divided into groups: normoxia, hypoxia, hypoxia followed by 1 day and 15 days in FiO2 0.21, and hypoxia pretreated with Src kinase inhibitor PP2, prior to hypoxia followed by 1 day and 15 days. Hypoxia was achieved by providing 7% FiO2 for 1 hour and PTP-1B expression was measured via immunoblotting. Results. PTP-1B increased posthypoxia by about 30% and persisted for 2 weeks while Src kinase inhibition attenuated the expected PTP-1B-increased expression. Conclusions. Our study suggests that Src kinase mediates a hypoxia-induced increased PTP-1B expression.