A Role for CD154, the CD40 Ligand, in Granulomatous Inflammation
Differential clearance of suture bundles in WT and CD154KO mice. Representative Masson’s trichrome-stained tissue sections from WT and CD154KO mice at week 10. (a and d) Low magnification (scale bar 500 μm); arrows indicate the nonresorbable thread which, upon section by the microtome, creates an artefactual void on the tissue section (asterisk); squares highlight the FBR in subcutaneous tissues. (b and e) High magnifications (scale bar 20 μm); the granulomas are composed of macrophages and multinucleated giant cells (MGCs) (arrows) bordered by fibrosis (green/blue). (c and f) Representative liver tissue sections from WT and CD154KO mice stained with the anti-F4/80 antigen monoclonal antibody at week 10; high magnifications (scale bar 20 μm), macrophages, and MGCs (arrows) express the F4/80 antigen macrophagic marker. (g) Quantification of granuloma areas in WT and CD154KO mice ().