Mediators of Inflammation / 2017 / Article / Fig 6

Research Article

A Role for CD154, the CD40 Ligand, in Granulomatous Inflammation

Figure 6

CD154 enhances phagocytosis by in vitro-derived macrophages. (a and b) CD40 is expressed by in vitro-derived macrophages and multinucleated giant cells (MGCs). (a) Macrophages derived from peripheral blood monocytes were immunostained for CD40 expression. Top panel, representative microphotographies of in vitro-derived macrophages (×100 and ×200 magnifications); bottom panel, CD40 immunostaining; nuclei were counterstained with DAPI (×400 magnification). (b) MGCs were obtained by IL-4-induced macrophage fusion and immunostained for CD40 expression. Top panel, representative microphotographies of in vitro-derived MGCs (×100 and ×200 magnifications); bottom panel, CD40 immunostaining; nuclei were counterstained with DAPI (×400 magnification). (c and d) CD154 enhances phagocytosis by in vitro-derived macrophages. (c) Cultures of in vitro-derived macrophages were assayed for phagocytosis in the presence or not of rsCD154. (d) Results obtained after fusion induction by IL-4. Cells were prestimulated 24 hours with 200 ng/mL rsCD154, and phagocytosis of opsonized erythrocytes was measured at various time-points (mean ± SD, ; ).
(a)
(b)
(c)
(d)