Effects of Secreted Mast Cell Mediators on Retinal Pigment Epithelial Cells: Focus on Mast Cell Tryptase

Figure 3

Antibody array analysis of culture supernatants from RPE cells stimulated by tryptase, histamine, or TNF-α. Representative arrays are shown. RPE cells were incubated in each medium for 24 h. (a) Incubation with only serum-free medium. (b) Incubation with serum-free medium containing tryptase (10 nM). (c) Incubation with serum-free medium containing histamine (200 μg/ml). (d) Incubation with serum-free medium containing TNF-α (10 ng/ml). Cells constitutively produced IL-8, MCP-1, and TIMP-2. Incubation with tryptase, histamine, or TNF-α enhanced IL-8 production (red square) and TNF-α also enhanced MCP-1 production (red square). (e) The mean optical intensity of IL-8 positive spots was assessed.

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