Effects of Secreted Mast Cell Mediators on Retinal Pigment Epithelial Cells: Focus on Mast Cell Tryptase

Figure 4

IL-8 production by RPE cells stimulated with mast cell mediators. ELISA showed constitutive IL-8 production by the cells. RPE cells were incubated with or without tryptase, histamine, TNF-α, eotaxin, MIP-1α, or I-309 at various concentrations for 24 h. IL-8 production was significantly enhanced by tryptase, histamine, or TNF-α in a concentration-dependent manner, while eotaxin, MIP-1α, and I-309 did not influence IL-8 production. Data are shown as the mean ± SEM of three independent experiments. , significantly different from the control.