Effects of Secreted Mast Cell Mediators on Retinal Pigment Epithelial Cells: Focus on Mast Cell Tryptase

Figure 7

Expression of phosphorylated ERK 1/2 by tryptase-stimulated RPE cells. Cell lysates were analyzed with an antibody array and representative results are shown. (a) Incubation with serum-free medium. (b) Incubation with tryptase (10 nM) for 1 h. Cells constitutively expressed phosphorylated-ERK 1/2, and tryptase stimulation increased phosphorylated-ERK 1/2 expression (red square). The mean optical intensity of positive spots was estimated (c).

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