Research Article
Molecular Responses of Human Retinal Cells to Infection with Dengue Virus
Table 1
Primer pairs and product sizes for gene transcripts.
| | Gene transcript | Primer pair | Product size (bp) |
| | CLDN5 [58] | Forward: 5-GCAGCCCCTGTGAAGATTGA-3
Reverse: 5-GTCTCTGGCAAAAAGCGGTG-3 | 106 | | CTNNB1 [59] | Forward: 5-AAAGCGGCTGTTAGTCACTGG-3
Reverse: 5-GACTTGGGAGGTATCCACATCC-3 | 132 | | DENV | Forward: 5-GCAGATCTCTGATGAATAACCAAC-3
Reverse: 5-TTGTCAGCTGTTGTACAGTCG-3 | 102 | | EIF2AK2 [60] | Forward: 5-GGAAAGCGAACAAGGAGTAAGG-3
Reverse: 5-CCAAAGCGTAGAGGTCCACT-3 | 97 | | GAPDH | Forward: 5-AGCTGAACGGGAAGCTCACTGG-3
Reverse: 5-GGAGTGGGTGTCGCTGTTGAAGTC-3 | 209 | | IFITM1 [61] | Forward: 5-ACTCCGTGAAGTCTAGGGACA-3
Reverse: 5-TGTCACAGAGCCGAATACCAG-3 | 155 | | IFN-β [62] | Forward: 5-AAACTCATGAGCAGTCTGCA-3
Reverse: 5-AGGAGATCTTCAGTTTCGGAGG-3 | 168 | | IL-6 [63] | Forward: 5-ATGAACTCCTTCTCCACAAGCGC-3
Reverse: 5-GAAGAGCCCTCAGGCTGGACTG-3 | 628 | | ISG15 [64] | Forward: 5-GAGAGGCAGCGAACTCATCT-3
Reverse: 5-AGCATCTTCACCGTCAGGTC-3′ | 99 | | JAM-3 [65] | Forward: 5-CCCTGTCTGTAGAGTGCCGAAG-3
Reverse: 5-GAGCCTGCGTCATTGGAAGC-3 | 258 | | OCLN [66] | Forward: 5-TGCATGTTCGACCAATGC-3
Reverse: 5-AAGCCACTTCCTCCATAAGG-3 | 235 | | PPIA | Forward: 5-GGCAAATGCTGGACCCAACACAAA-3
Reverse: 5-CTAGGCATGGGAGGGAACAAGGAA-3 | 355 | | RSAD2 [67] | Forward: 5-TGACGGAACAGATCAAAGCA-3
Reverse: 5-GCACCAAGCAGGACACTTCT-3 | 174 | | TBP [68] | Forward: 5-GCCTCCCCCACCCCCTTCTTT-3
Reverse: 5-GCCACACCCTGCAACTCAACATCC-3 | 106 | | TNF-α [63] | Forward: 5-TCTCGAACCCCGAGTGACAA-3
Reverse: 5-TGAAGAGGACCTGGGAGTAG-3 | 181 | | VE-cadherin [69] | Forward: 5-GGGTTTTTGCATAATAAGCAGG-3
Reverse: 5-GCACCAGTTTGGCCAATATA-3 | 149 | | ZO-1 [70] | Forward: 5-GAACGAGGCATCATCCCTAA-3
Reverse: 5-CCAGCTTCTCGAAGAACCAC-3 | 218 |
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References for primers sequences sourced from the literature are provided. For primer pairs designed in-house, products were confirmed by sequencing. B2M: β-2-microglobulin; CLDN5: claudin 5; CTNNB1: catenin-β1; DENV: dengue viral RNA; EIF2AK2: eukaryotic translation initiation factor 2-alpha kinase 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IFITM1: interferon-induced transmembrane protein 1; IFN-β: interferon-β; IL-6: interleukin-6; ISG15: interferon-stimulated gene 15; JAM-3: junctional adhesion molecule 3; OCLN: occludin; PPIA: peptidylprolyl isomerase A; RSAD2: radical SAM domain-containing 2 (viperin); TBP: TATA-binding protein; TNF-α: tumor necrosis factor-α; ZO-1: zonula occludens 1.
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