Research Article

Inhibiting Interleukin 17 Can Ameliorate the Demyelination Caused by A. cantonensis via iNOS Inhibition

Figure 4

IL-17A inhibition causes the downregulation of iNOS in astrocytes after A. cantonensis infection. (a) The RNA expression of IL-17A and iNOS in the normal group, the 21 d A. cantonensis infection group, the normal mice with IL-17A antibody group, and the 21 d A. cantonensis infection with IL-17A antibody group. After IL-17A antibody was injected, the RNA expression of IL-17A was decreased in the A. cantonensis-infected group on day 21 but it had no effect on the normal group. IL-17A antibody increased the RNA expression of iNOS in the normal group, but levels were decreased in the 21 d A. cantonensis infection group. GAPDH was the internal reference for real-time PCR. (b) Brain sections stained with iNOS (red) and GFAP (green). Yellow color represented the double staining of iNOS and GFAP. (c) Percentage of iNOS+/GFAP+ cells per field of the brain section. , , compared with the normal group; #, ###, compared with the 21 d A. cantonensis infection group. mice.
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