FTY720 Attenuates Angiotensin II-Induced Podocyte Damage via Inhibiting Inflammatory Cytokines

Figure 2

FTY720 could protect Ang II-induced kidney tissue injury in rats. The rats were subjected to subcutaneously continuous Ang II-infusion (400 ng/kg⋅min) for 14 or 28 days and were sacrificed. Rats given FTY720 (0.5 g/kg⋅d gavage) for 14 or 28 days also received Ang II-infusion. After the mice were sacrificed, the kidneys were fixed in paraformaldehyde for HE staining. The other parts of renal cortex were fixed in glutaraldehyde and podocytes were analyzed using transmission electron microscope. Kidney homogenate was used for RIA analysis. The plasma and serum were separated for biochemical analysis. Data represented is means ± SD of six independent experiments performed in triplicate. () , versus normal saline infusion control rats; ^#, versus Ang II-infusion rats. (a) Serum creatinine levels in Ang II and FTY720-treated rats. (b) Serum urea nitrogen levels in Ang II and FTY720-treated rats. (c) Serum albumin levels in rats treated with Ang II and FTY720 for 14 and 28 days. (d) Concentration of Ang II in plasma from rats treated with Ang II and FTY720 for 14 and 28 days. (e) Concentration of Ang II in renal tissue homogenate from rats treated with Ang II and FTY720 for 14 and 28 days. (f) Twenty-four-hour urinary protein excretion levels in rats treated with Ang II and FTY720 for 14 and 28 days. (g) Light microscopy evaluation of rat kidney pathological changes with hematoxylin and eosin (H&E) staining (magnification, ×200). (h) Transmission electron microscopy evaluation of micrographs of podocyte ultrastructure in rats (magnification, ×8000).

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