Cannabinoid Receptor 2 Modulates Neutrophil Recruitment in a Murine Model of Endotoxemia
CB2 deficiency results in higher MMP-9 levels in the serum of endotoxemic mice. Male C57BL/6J and CB2 knockout mice (8–10 weeks old) were administered i.p. with 1 mg/kg LPS and the levels of proinflammatory mediators in the serum at 2 hours was measured. The levels of TNF-α (a), IL-6 (b), CCL2 (c), CCL3 (d), CCL4 (e), CXCL1 (f), CXCL5 (g), CXCL10 (h), and MMP-9 (i) were measured in serum samples by Luminex. Data are from two independent experiments with 6–9 mice per group and 3–5 mice per group per experiment. Mean + SEM are represented in all bar graphs and data were analysed with a one-tailed student t-test, .