Elevated Glucose and Interleukin-1β Differentially Affect Retinal Microglial Cell Proliferation

Figure 5

High glucose and IL-1β have opposite effects on retina microglial and macroglial cell proliferation. Rat retinal neural cell cultures were exposed to high glucose for 7 days or IL-1β for 24 h. Iba-1 immunoreactivity was assessed by immunocytochemistry (a, b). GFAP immunoreactivity was assessed by immunocytochemistry (c, d) or Western blotting (e). ED-1 immunoreactivity was assessed by immunocytochemistry (f) or Western blotting (g). Representative images (insets of higher magnifications images are shown below each panel) for Iba-1 (a), GFAP (c), or ED-1 (f) upon high glucose and IL-1β treatment are shown. Scale bar: 100 μm for (c). Scale bar: 50 μm for (a) and (f). Data represent means ± SEM of at least 4 independent experiments. , , significantly different from control as determined by ANOVA followed by Dunnett’s post hoc test.

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