Table of Contents Author Guidelines Submit a Manuscript
Mediators of Inflammation
Volume 2017 (2017), Article ID 4327237, 7 pages
Research Article

Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study

1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of Korea
2Department of Pharmacology, Yonsei University College of Medicine, Seoul, Republic of Korea
3Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Republic of Korea
4The Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul, Republic of Korea
5The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Republic of Korea

Correspondence should be addressed to Hyung-Ju Cho

Received 14 March 2017; Revised 20 June 2017; Accepted 2 August 2017; Published 6 September 2017

Academic Editor: Sandra Helena Penha Oliveira

Copyright © 2017 Eun Jung Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. We hypothesized that CIH may affect the upper airway immune system and aimed to verify whether CIH can induce airway inflammation in a murine obstructive sleep apnea (OSA) model. Methods. C57BL6 male mice were exposed to intermittent hypoxia (CIH group; 5 ~ 21% FiO2, 120 sec cycles, 12 h/d, ) or room air (Sham group, ) for up to 4 weeks in identical chambers. Nasal and lung tissues and lavage fluid were collected and analyzed by multiplex assay. Lung lavage fluid was also utilized for FACS analysis to determine eosinophil count. Results. We determined the protein level of 24 different cytokines, chemokines, and inflammatory mediators. Among various cytokines, levels of IL-1α, IL-1β, IL-4, IL-6, and IL-13 were significantly elevated in nose or lung tissue from the CIH group. In addition, MCP-1 and periostin were elevated in nose and lung tissue and lavage fluid from the CIH group. Conclusions. CIH for 4 weeks altered the levels of inflammatory mediators in both the nose and lungs of mouse model. We suggest that the airway immune system may be deteriorated by CIH and allergic inflammation in the upper or lower airway could be worsened by sleep apnea.