Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis

Figure 3

SF-derived exosomes stimulate the production of several cytokines by M1 macrophages. (a) M1 macrophages, differentiated from normal donor monocytes, were incubated with SF-derived exosomes for 6 hours and cytokine expression was analysed by RT-PCR. Data were expressed as fold change relative to unstimulated cells. (b) M1 macrophages were treated with SF-derived exosomes (b) or IFN-γ/LPS (c) for 24 hours, and cytokine production was quantified in supernatants by ELISA Bio-plex cytokine assay system. Data were expressed as fold change relative to unstimulated cells. Columns, mean; bars, SD, significant difference from unstimulated cells .

(a)

(b)

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