Research Article
Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells
Figure 5
Effects of OF and CM on lipid deposit in oleic acid- (OA-) stimulated mesangial SV40-Mes13 cells. Mesangial cells were incubated with 200 μM OA/BSA and cotreated with various concentrations of OF and CM for 12 h. Vehicle cells were incubated with 0.1% DMSO in the presence of OA/BSA. Control cells were incubated with 1% BSA. Cell viability was measured by the Alamar blue assay (a). Quantitative analysis of lipid deposition in cells by the OD500 nm values using Oil Red O staining (b). Intracellular triglyceride (c) and cholesterol (d) contents were determined in cell lysates by an enzymatic colorimetric method using a commercially available kit. Total cholesterol and TG contents of the control cells were 9.8 ± 0.1 and 7.5 ± 0.2 μg/mg cellular protein, respectively. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ().
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