Group 2 Innate Lymphoid Cells Are Involved in Skewed Type 2 Immunity of Gastric Diseases Induced by Helicobacter pylori Infection

Figure 3

GATA-3 was enhanced in lymphocytes isolated from H. pylori⁺ gastric cancer tissues, which was responsible for the skewed type 2 immune response. As shown in the immunohistochemical staining, when compared to the H. pylori⁻ gastric cancer tissues (a), GATA-3 expression was significantly upregulated in the interstitial tissues of H. pylori⁺ gastric cancer tissues (b). Moreover, the GATA-3⁺ cell indicated a morphological characteristic of lymphocyte. (c, d) Tumor-infiltrating lymphocytes from H. pylori⁺ gastric cancer tissues (Hp⁺) showed a stronger secretion of IL-4 but a weaker secretion of IFN-γ, when compared to those from H. pylori⁻ gastric cancer tissues (Hp⁻). (e–i) After preparation of mononuclear cells from gastric tissues, GATA-3⁺ population was found to increase in H. pylori⁺ gastric cancer tissues by flow cytometry. Moreover, Lin⁻GATA-3⁺ population and Lin⁺GATA-3⁺ population were increased in cells from H. pylori⁺ gastric cancer tissues, respectively. , .

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