Mediators of Inflammation / 2017 / Article / Fig 5

Research Article

Nuclear Translocation of SGPP-1 and Decrease of SGPL-1 Activity Contribute to Sphingolipid Rheostat Regulation of Inflammatory Dendritic Cells

Figure 5

(a) Quantitative real-time PCR for abcc1, abca1, and abcg1 in LPS-stimulated dendritic cells over time normalized to the sum of csnk2a2 and fbxo38 mRNA levels. Levels at time point 0 were set to 1 for each mRNA (). (b) Western blot stained with anti-Abcc1 antibody of whole-cell lysates of naïve dendritic cells or 48 h upon LPS stimulation. Quantification of Western blot bands (). (c) Quantitative real-time PCR for abcc1, abca1, and spns2 in untreated dendritic cells (). Displayed are absolute levels normalized to the sum of csnk2a2 and fbxo38 mRNA levels. (d) Quantitative real-time PCR for spns2 in LPS-stimulated dendritic cells over time normalized to the sum of csnk2a2 and fbxo38 mRNA levels. Levels at time point 0 were set to 1 (). Data represent mean ± SD.
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